Capillary electrophoresis is a technique originating from plate electrophoresis. The application of siliceous capillaries with a small diameter allowed to automate the analytical process while increasing sensitivity, accuracy and resolution. The electrophoretic separation occurs on the basis of difference in the migration speed of charged particles in a constant electric field.
We have three Beckman systems allowing us to carry out capillary electrophoresis: PA800, PA800 plus and P/ACE MDQ equipped with UV, PDA and LIF detectors. Devices are equipped with an autosampler with a cooling option. The capillary is thermostated with a cooling liquid within the range of 15°C to 60°C. The separation takes place in the siliceous capillaries with an inner diameter of 50 or 75 µm and the length ranging from 20 to 50 cm with the maximum level of voltage at 30 kV, in the mode of normal or reversed polarisation. Systems are automatically managed by a computer with the installed 32 Karat software. The tools allow carrying out the analyses with the use of three techniques: free zone capillary electrophoresis, capillary gel electrophoresis and isoelectric focusing.