The gel plate electrophoresis is a traditional electrophoretic technique that is applied in the qualitative analysis of protein mixtures. The separation of particles takes place in the agarose or in the polyacrylamide gel. Due to composition of the buffer that is used during electrophoresis, the analysis can be carried out in the denaturing (SDS-PAGE) or non-denaturing (native electrophoresis) conditions.
We also have the Agilent 2100 Bioanalyzer system which allows us to carry out the electrophoresis on chips. It is a miniaturized, fully automated version of the traditional plate electrophoresis which allows qualitative and quantitative analysis of the protein mixtures. The main benefits of this technique include an increased sensitivity, accuracy and the possibility to visualize the results in the form of electrophoregrams.
Capillary electrophoresis is a technique that stems from the plate electrophoresis. The application of siliceous capillaries with a small diameter allows automating the analytical process, increasing sensitivity, accuracy and resolution. The electrophoretic separation takes place on the basis of differences between the speed of particle migration with positive charge in a static magnetic field.
We have three systems for capillary electrophoresis manufactured by Beckman: PA800, PA800 plus and P/ACE MDQ equipped with UV detectors, PDA and LIF. The units are also equipped with an autosampler which features the cooling option. The capillary is equipped with a thermostat as well as cooling liquid within the range of 15°C up to 60°C. Separation takes place in the siliceous capillaries with an inner diameter of 50 or 75 µm and the length of 20 to 50 cm with the maximum level of voltage at 30 kV, in the normal or reversed polarization mode. Systems are automatically managed by a computer with the installed 32 Karat software. These devices allow us carry out the analysis using three techniques: free zone capillary electrophoresis, capillary gel electrophoresis and isoelectric focusing.