We offer the possibility to prepare starters on the basis of the provided sequences of the desired DNA insert and on the basis of the provided biological material, amplification reactions of the desired DNA fragment, all the necessary DNA purification stages as well as the introduction of the eukaryotic expression vector. Genetic constructs will be prepared on the basis of the commercially available vectors and on the basis of our own patented cloning systems developed at IBA.

Expression of recombinant human bifunctional peptidylglycine α-amidating monooxygenase in CHO cells and its use for insulin analogue modification.

Zieliński M, Wójtowicz-Krawiec A, Mikiewicz D, Kęsik-Brodacka M, Cecuda-Adamczewska V, Marciniak-Rusek A, Sokołowska I, Łukasiewicz N, Gurba L, Odrowąż-Sypniewski M, Baran P, Płucienniczak G, Płucienniczak A, Borowicz P, Szewczyk B. Protein Expr Purif. 2016 Mar;119:102-9. Epub 2015 Nov 22.

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ATTENTION! The material provided by the customer:

  • matrix DNA or its sequence only,
  • information about the required benchmark sequence,
  • expression bacteria provided by the customer (in the situation of introducing to expression bacteria other than those used at IBA),
  • expression vectors provided by the customer (in the situation of introducing to the expression bacteria other than those used at IBA).

Planned duration: as agreed with the customer.

High resolution 3d render of human dna string

Contact

Diana Mikiewicz, M.Sc.
Phone: + 48 22 37 86 226
mikiewiczd@iba.waw.pl

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Luiza Chojnacka-Puchta, Ph.D.
Phone: + 48 22 86 226
chojnackal@iba.waw.pl

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Anna Wójtowicz-Krawiec, Ph.D.
Phone: + 48 22 37 86 227
wojtowicza@iba.waw.pl